Lab-Tacular Tools

Solve C1V1 = C2V2 for concentration or volume.

Molecular weight and per-element composition from a chemical formula.

Solve for mass, molecular weight, volume, or molarity from the other three.

Calculates the concentration at each step of a serial dilution, and the volume to transfer.

Calculates the stock and diluent volume needed for a target concentration and volume.

Works out the volume to add to your medium, from the standard concentrations for nine antibiotics.

Calculates nucleic acid concentration and purity ratios from A260.

Works out the volume to pool from each library, from its length and concentration.

Paste an oligo list and get the buffer volume for every tube.

Calculates the mean, standard deviation and CV (%) from your measurements.

Fits a standard curve and back-calculates unknown concentrations.

Fits a four-parameter logistic (4PL) and reads sample concentrations back off it.

Calculates qPCR amplification efficiency (%) from a standard curve slope.

Works out how much of each component a batch of reactions needs.

Finds a buffer's pH, or the amounts to weigh out for a target pH.

Turns a gel volume and target percentage into agarose, buffer and stain amounts.

Works out the resolving and stacking gel recipes from a percentage and a gel count.

Turns haemocytometer counts into cell concentration and viability.

Works out the seeding volume for each vessel, from your current density and viability.

Converts between %(w/v) concentration and molarity.

Converts between molarity and mg/mL through the molecular weight.

Converts between RCF and RPM using rotor radius.

Converts between ng, pmol and copy number from nucleic acid length.

Turns a length in bp or nt into a molecular weight and the mass of one picomole.

Turns an A280 reading into mg/mL using your extinction coefficient.

Converts between absorbance (A) and transmittance (%T).

Works out pH, [H⁺], pOH and [OH⁻] from any one of them.

Converts a temperature between Celsius, Fahrenheit and kelvin.

Converts a pressure between Pa, bar, psi, atm and mmHg.

Converts an energy between J, cal, kcal and eV.

Calculates fold changes against a control group from qPCR Ct values.

Normalises band densities and works out the fold change against the control.

Draws an error bar chart from grouped data, with the summary statistics.

Works out what goes in a ligation reaction from the molar ratio you want.

Calculates transformation efficiency in cfu/µg from colony counts.

Works out how many subjects an experimental group needs for a given power.

Finds the growth rate and doubling time from a time course.

Gets the IC50 and the Hill slope from dose–response data.

Km and Vmax from substrate concentrations and initial rates.

Picks the statistical test that fits your data, and runs it.

Turns your QC values into control limits and a Westgard rule verdict.

Tests whether the most extreme value in a set of replicates is an outlier.